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Infrared LED oblique illumination for studying neuronal circuits

Difficulty level
Beginner
Speaker
Duration
25:16
Topic

This lecture covers infrared LED oblique illumination for studying neuronal circuits in in vitro block-preparations of the spinal cord and brain stem.

Topics covered in this lesson
  • Organization of the spinal dorsal horn
  • Methods used to study the organization, function, and connectivity of the spinal dorsal horn
  • Introduction to the infrared LED oblique illumination technique for studying neuronal circuits in the spinal cord
  • Demonstration of the spinal cord block preparation (7:27)
  • Demonstration of removing the meninges from the spinal cord (12:37)
  • Demonstration of how to fix the spinal cord preparation to a gold plate (13:46)
  • Equipment required to perform the infrared LED oblique illumination technique (23:23)