In vivo optogenetics
In vivo optogenetics
This lecture provides an introduction to optogenetics, a biological technique to control the activity of neurons or other cell types with light.
Topics covered in this lesson
- Objectives of optogenetics
- Challenges of manipulating neural circuits (0:34)
- Methods to change activity in genetically identified neurons (9:17)
- Types of opsins used in optogenetics (12:37)
- Illumination of channelrhodopsin in epressed in the motor cortex (16:49)
- Expression of halorhodopsin in human photoreceptors (20:19)
- Studies enabled by optogenetics (21:05)
- Limitations of optogenetics (29:03)
- Methods to overcome the limitations of optogenetics (32:54)
- Two-photon optogenetics (34:14)
- How activity in neural circuits gives rise to perception and action (35:12)
- Combining two-photon calcium imaging and spatial light modulator (SLM)-based two-photon optogenetics (36:10)
External links