Functional Fluorescence Microscopy Imaging (fFMI) in Biomedical Research
This course is a basic course on advanced fluorescence microscopy imaging and correlation spectroscopy techniques for quantitative characterization of molecular transport and interactions in live cells. The purpose of the course is to give an introduction of the underlying physicochemical principles, hands-on training and an overview of applications of these specialized techniques in biomedical research. At the end of the course, the student will have hands-on experience with live-cell imaging and specialized fluorescence microscopy and correlation spectroscopy techniques. The course is suitable for doctoral students lacking training in mathematics, physics, or optical engineering who want to apply these techniques in their research.
- Use fundamental aspects of molecular structure to explain light-matter interactions and the emission of fluorescence. Use this knowledge to discuss fluorescent properties of different types K8F2348 Functional Fluorescence Microscopy Imaging (fFMI) in Biomedical Research, 3 higher education credits (hec) / Funktionell Fluorescens Mikroscopi Avbildning (fFMA) i biomedicinsk forskning, 3 högskolepoäng Third-cycle level / Forskarnivå of fluorophores
- Compare the buildup of different types of fluorescence microscopes. Identify different optical elements and explain their function
- Assess the strengths and weaknesses of different fluorescence labeling procedures and justify the choice in relation to the biological question studied
- Explain the theoretical background behind specialized fluorescence microscopy based methods for studying molecular interactions in live cells. Discuss pros and cons in relation to the biological question studied
- Explain the theoretical background behind different super-resolution microscopy techniques. Discuss pros and cons in relation to the biological question studied
- Assess the adequateness of the fluorescence microscopy based technique used in the scientific literature concerned. Evaluate pros and cons in relation to the biological question studied
- Select an appropriate fluorescence microscopy based technique for a biological question of interest, justify the choice and specify instrumental requirements
No prerequisite courses, or equivalent, demanded for this course.