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About this course

This course is a basic course on advanced fluorescence microscopy imaging and correlation spectroscopy techniques for quantitative characterization of molecular transport and interactions in live cells. The purpose of the course is to give an introduction of the underlying physicochemical principles, hands-on training and an overview of applications of these specialized techniques in biomedical research. At the end of the course, the student will have hands-on experience with live-cell imaging and specialized fluorescence microscopy and correlation spectroscopy techniques. The course is suitable for doctoral students lacking training in mathematics, physics, or optical engineering who want to apply these techniques in their research.

Learning outcomes
  1. Use fundamental aspects of molecular structure to explain light-matter interactions and the emission of fluorescence. Use this knowledge to discuss fluorescent properties of different types K8F2348 Functional Fluorescence Microscopy Imaging (fFMI) in Biomedical Research, 3 higher education credits (hec) / Funktionell Fluorescens Mikroscopi Avbildning (fFMA) i biomedicinsk forskning, 3 högskolepoäng Third-cycle level / Forskarnivå of fluorophores
  2. Compare the buildup of different types of fluorescence microscopes. Identify different optical elements and explain their function
  3. Assess the strengths and weaknesses of different fluorescence labeling procedures and justify the choice in relation to the biological question studied
  4. Explain the theoretical background behind specialized fluorescence microscopy based methods for studying molecular interactions in live cells. Discuss pros and cons in relation to the biological question studied
  5. Explain the theoretical background behind different super-resolution microscopy techniques. Discuss pros and cons in relation to the biological question studied
  6. Assess the adequateness of the fluorescence microscopy based technique used in the scientific literature concerned. Evaluate pros and cons in relation to the biological question studied
  7. Select an appropriate fluorescence microscopy based technique for a biological question of interest, justify the choice and specify instrumental requirements
Prerequisites for participation

No prerequisite courses, or equivalent, demanded for this course. 

Necessary language skill
English
Semester(s) in which the module takes place
Autumn 2024
Course level
Doctoral
Course start date
11 Nov 2024
Course end date
22 Nov 2024
Apply between
Application start date
15 Apr 2024
-
Application end date
30 Sep 2024
Teaching mode
Local
Workload in ECTS
3.0
Graded
Yes
Person in charge of module
Vladana Vukojevic
Email address
vladana.vukojevic@ki.se
Local module ID
K8F2348
Documents
Course Syllabus (234.95 KB)