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Imaging Synapses at Nanoscale Resolution

University of Bonn
Empirical & clinical neuroscience
About this course

Topics covered include:

  • Basic fluorescence microscopy
  • Fluorescence lifetime imaging (FLIM), 2P excitation
  • Fluorescence resonance energy transfer (FRET)
  • Stochastic optical reconstruction microscopy (d-STORM)
  • 3D-Electron microscopy, focused-ion beam (FIB) milling and scanning EM, specimen preparation and embedding.
Learning outcomes

Introduction to novel imaging techniques and modalities revealing the nanostructural architecture of synapses.

Prerequisites for participation

45 ECTS

Necessary language skill
English
Semester(s) in which the module takes place
Winter Semester
Course type
Practical + Seminar
Course level
Masters
Course start date
06 Jan 2024
Course end date
28 Feb 2024
Involvement period
8 weeks
Teaching mode
Local
Local attendance
Required
Workload in ECTS
15
Graded
Yes
Person in charge of module
Prof. Dr. Dirk Dietrich
Local module ID
WPP 34