Skip to main content

Translational neurooncology

University of Bonn
Empirical & clinical neuroscience
About this course

Topics covered include:

  • Basics of monolayer glioblastoma cell culturing
  • Generation and culturing of human glioblastoma organoids
  • Immunochemistry, Western Blot, DNA/RNA isolation
  • Flow cytometry analysis of cell death and cell viability assays
  • Live-cell imaging and morphology analysis
  • Data analysis including statistics using ImageJ, FlowJo, GraphPad PRISM
  • Visualization of results using Microsoft Excel/Powerpoint or Adobe illustrator
Learning outcomes

Students should gain insights into preclinical pharmacological studies in glioblastoma research. They will learn basic principles of culturing glioblastoma cell populations and organoids and will have the possibility to generate cell populations and organoids from fresh tumor material from glioblastoma material obtained from the operating room. Additionally, students will develop skills in experimental planning and fundamental laboratory techniques including immunofluorescence, western blotting, cell viability assessment and flow cytometric assessment of cell death. Live-cell
imaging under treatment and subsequent morphology analysis of glioblastoma cells will also be conducted. Using these techniques, they will explore the effects of various drugs and drug combinations, including chemotherapy and gap junction
inhibitors on glioblastoma cells and organoids.

Prerequisites for participation

45 ECTS

Necessary language skill
English
Interval at which Module/Course is offered
Summer and Winter Semesters
Semester(s) in which the module takes place
Winter Semester
Course type
Practical + Seminar
Course level
Masters
Course start date
06 Jan 2025
Course end date
28 Feb 2025
Teaching mode
Local
Local attendance
Required
Workload in ECTS
15
Graded
Yes
Person in charge of module
PD Dr. med. Matthias Schneider and Dr. med. Anna-Laura Potthoff
Local module ID
WPP 63