Topics covered include:
- Basics of monolayer glioblastoma cell culturing
- Generation and culturing of human glioblastoma organoids
- Immunochemistry, Western Blot, DNA/RNA isolation
- Flow cytometry analysis of cell death and cell viability assays
- Live-cell imaging and morphology analysis
- Data analysis including statistics using ImageJ, FlowJo, GraphPad PRISM
- Visualization of results using Microsoft Excel/Powerpoint or Adobe illustrator
Students should gain insights into preclinical pharmacological studies in glioblastoma research. They will learn basic principles of culturing glioblastoma cell populations and organoids and will have the possibility to generate cell populations and organoids from fresh tumor material from glioblastoma material obtained from the operating room. Additionally, students will develop skills in experimental planning and fundamental laboratory techniques including immunofluorescence, western blotting, cell viability assessment and flow cytometric assessment of cell death. Live-cell
imaging under treatment and subsequent morphology analysis of glioblastoma cells will also be conducted. Using these techniques, they will explore the effects of various drugs and drug combinations, including chemotherapy and gap junction
inhibitors on glioblastoma cells and organoids.
45 ECTS