Topics covered include:

• CRISPR/Cas9 mediated genome editing in mammalian cell lines
• CRISPRoff genome editing tools to modify activity of gene promoters
• Cloning of promoter regions and relevant proteins into reporter gene and mammalian expression vectors
• Reporter gene assays to measure activity of gene promoters or unknown DNA sequences using plate luminometer
• Chemical modification of genomic DNA for DNA methylation analysis
• Pyrosequencing for detection and quantification of DNA methylation
• Chromatin preparation and chromatin immunoprecipitation analysis
• Standard PCR and quantitative reverse transcription PCR analysis
• Transfection and expression of relevant proteins in mammalian cells
• Western blotting for protein analysis