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Functional Characterization of Neuronal Cell Types

University of Bonn
Empirical & clinical neuroscience
About this course

Topics covered include:

  • Diversity of neuronal cell types and approaches to cell type classification
  • Experimental design to dissect the functional contribution of diverse neuronal cell types to behaviour
  • Stereotaxic surgeries and cell type-specific targeting with viral vectors in transgenic mice
  • Deep-brain imaging at the single-cell level using miniature microscopes in freely-moving mice and 2-photon recordings in head-fixed animals
  • All-optical interrogation of neural circuits with combined imaging and optogenetic approaches
  • Introduction to analysis of deep-brain imaging data
Learning outcomes

Students will be introduced to different concepts of neuronal cell type diversity (molecular, anatomical, functional). They will gain knowledge about state-of-the-art techniques used for dissecting the contribution of diverse neuronal cell types to
behaviour. Students will gain hands-on experience with imaging techniques at the single-cell level in behaving mice and related data analysis.

Prerequisites for participation

45 ECTS

Necessary language skill
English
Semester(s) in which the module takes place
Winter Semester
Course type
Practical + Seminar
Course level
Masters
Course start date
06 Jan 2025
Course end date
28 Feb 2025
Teaching mode
Local
Local attendance
Required
Workload in ECTS
15
Graded
Yes
Person in charge of module
Dr. Sabine Krabbe
Local module ID
WPP 44