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About this course

Topics covered include:

  • CRISPR/Cas9 mediated genome editing in mammalian cell lines
  • CRISPRoff genome editing tools to modify activity of gene promoters
  • Cloning of promoter regions and relevant proteins into reporter gene and mammalian expression vectors
  • Reporter gene assays to measure activity of gene promoters or unknown DNA sequences using plate luminometer
  • Chemical modification of genomic DNA for DNA methylation analysis
  • Pyrosequencing for detection and quantification of DNA methylation
  • Chromatin preparation and chromatin immunoprecipitation analysis
  • Standard PCR and quantitative reverse transcription PCR analysis
  • Transfection and expression of relevant proteins in mammalian cells
  • Western blotting for protein analysis
Learning outcomes

Students receive hands-on experience into current molecular and cell biological methods for the detection of epigenetic modifications and the functional analyses of epigenetically regulated genes in cell models of neurodegeneration and glioblastoma.

Prerequisites for participation

45 ECTS

Necessary language skill
English
Semester(s) in which the module takes place
Winter Semester
Course type
Practical + Seminar
Course level
Masters
Course start date
28 Oct 2024
Course end date
20 Dec 2024
Involvement period
8 weeks
Teaching mode
Local
Local attendance
Required
Workload in ECTS
15
Graded
Yes
Person in charge of module
PD Dr. Bernd Evert
Local module ID
WPP 56